Fermentas is a wholly owned subsidiary of Thermo Fisher Scientific, and is a world leader in the discovery, manufacturing and marketing of quality molecular biology products. With more than 35,000 employees around the world, Thermo Fisher Scientific is the world leader in serving science, and the largest provider of analytical instruments, equipment, reagents and consumables, software and services for research, analysis discovery and diagnostics.

Fermentas has developed a proprietary patent-pending plasmid DNA preparation technology that allows preparation of various grades of plasmid DNA from milligram to multigram quantities suitable for various applications including standard manipulations, transfections and pre-clinical trials in gene therapy and vaccination. The pharmaceutical-grade plasmid DNA is characterized by significantly lower levels of contaminants and endotoxins (10 EU/mg) compared to one obtained using technologies currently available on the market. The pharmaceutical-grade plasmid DNA meets all EU and FDA guidelines for pharmaceuticals and has EU GMP certificate.

Research Grade Plasmid DNA

For standard DNA manipulations including re-cloning, sequencing and transformation

• Turnaround time – 15 working days or less
• Certificate of Analysis included

Transfection Grade Plasmid DNA

For transfection in vitro and in vivo

• Endotoxin levels below 0.01 EU/µg
• Free of organic solvents and mutagenic ethidium bromide
• Free of substances of animal origin
• Turnaround time – 15 working days or less
• Certificate of Analysis included

Pharmaceutical Grade Plasmid DNA

For pre-clinical trials in gene therapy/vaccination

• RNA and genomic DNA content extremely low
• Host cell proteins content below 10 ng/mg DNA
• Endotoxin levels below 0.01 EU/µg
• Free of organic solvents and mutagenic ethidium bromide
• Free of substances of animal origin
• Turnaround time – one month
• Tested according to EU CPMP/BWP/3088/99 and FDA guidelines
• Certificate of Analysis included

Introductory Offer (multicopy plasmids):

Research grade plasmid DNA
#SD0713, up to 400 mg
#SD0714, up to 1500 mg

Transfection grade plasmid DNA
#SD0722, 2-10 mg
#SD0723, 30 L fermentation (up to 400 mg)
#SD0724, 100 L fermentation (up to 1500 mg)

Pharmaceutical grade plasmid DNA*
#SD0731, 5-10 mg
#SD0732, 30 L fermentation (up to 400 mg)
#SD0733, 100 L fermentation (up to 1500 mg)

Additional Post-production Services

• Restriction enzyme** digest analysis
• Restriction enzyme digest
• Restriction enzyme digest and DNA fragment purification
• Sequencing
• Plasmid DNA archiving

* tested according to FDA and EU CPMP/BWP/3088/99 guidelines
** Restriction enzyme digests are performed with Fermentas enzymes all of which meet the PureExtreme® standard of quality.

Please contact fermentas.oem@thermofisher.com to receive a price quote for any of your current or upcoming projects.

Please click to see a sample Certificate of Analysis, pdf format 126 KB and the sample of Plasmid DNA Specification, pdf format 100 KB.